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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Dev Biol. 2012 Aug 28;372(1):103–110. doi: 10.1016/j.ydbio.2012.08.013

Fig. 4.

Fig. 4

Ryr is required for embryonic movement in X. laevis. (A) Schematic showing the binding site for the Ryr splice-blocking morpholino (RyrMO); numbers indicate expected PCR product size with indicated primers (arrows). (B) RT-PCR analysis of stage 27 embryos injected with either RyrMO or a 5 base pair mismatch Ryr morpholino (Ryr5MM). (C) Western blot analysis using an anti-Ryr antibody. β-tubulin was used as a loading control. (D) Ryr knockdown leads to complete paralysis in Xenopus embryos. Graph depicting percentage of touch-unresponsive stage 27 embryos injected with 20ng (RyrMO/embryo (n=23); these animals showed no spontaneous movement at later stages (data not shown). Control embryos were injected with 20ng Ryr5MM/embryo, and were largely responsive to touch (n=17). (E) Uninjected (UI), Ryr5MM, RyrMO and Eif4a3MO-injected embryos at stage 32.