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Eif4a3 associates with ryr mRNA. Cell extracts from stage 27 embryos injected with Myc-Eif4a3 (A) or Myc-Eif4a3 with Eif4a3MO (B) were incubated overnight with anti-Myc antibody or mouse IgG. Following immunoprecipitation, Myc-Eif4a3-associated RNA was purified, reverse transcribed, and amplified using gene-specific primers. “Input RNA”: non-immunoprecipitated RNA.
