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TRBP affects the kinetics of pre-miR processing by Dicer. (A) The predicted secondary structure of pre-let-7a and pre-miR-21. (B) Pre-let-7a and pre-miR-21 processing by Dicer and Dicer–TRBP in single turnover conditions ([Dicer] = 400 nM, [Dicer–TRBP] = 50 nM, [RNA] = 2 nM) and in multiple turnover condition ([Dicer], [Dicer–TRBP] = 5 nM, [RNA] = 50 nM; graphs represent data from three experimental replicates).
