Figure 3. Propofol inhibits the acquisition of neuronal polarity.
A: Cortical neuron cultures were treated with 3 μM propofol for four hours immediately after plating and assayed for neuronal polarity on DIV1. Similarly to isoflurane, propofol significantly reduces the mean percentage of polarized neurons. B: The GABAAR agonist muscimol does not alter mean percentage of polarized neurons in the same exposure paradigm, suggesting that the effects of isoflurane and propofol on axon specification do not occur via activity at GABAARs. n = 666 cells in 61 fields for A and n = 527 cells in 60 fields for B. * = p<0.05