(A–B) 13C isotopomer analysis of uniformly labeled palmitate (U13C-Palmitate). (A) Schematics depicting the number of carbons labeled (filled circles) in a defined set of metabolites derived from palmitate. Metabolites marked in red are selectively elevated in OxPhos-DLBCL cell lines (B). (B) 13C enrichment in palmitate-derived metabolites. For each metabolite, cumulative data obtained from all 4 OxPhos-DLBCL cell lines are shown relative to the mean value of that metabolite in all 4 BCR-DLBCL cell lines listed on top.
(C) Effect of palmitate supplementation on the proliferation of DLBCL cell lines. Control denotes serum-free media containing all amino acids except L-glutamine.
(D) Survival of DLBCL subsets cultured in the absence or presence of BrCA for 24 hr.
Error bars, ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; two-tailed Student’s t-test.
See also Figure S3.