Fig. 5.

In vivo binding of EpaA domains and the distribution of mucin-derived glycan ligands. (A) Relative adhesion to epithelial cells as conferred by different EpaA domains was determined by using a heterologous S. cerevisiae expression system. S. cerevisiae strains presenting different EpaA domains on Flo11 BC stalks were incubated with a monolayer of Caco-2 cells and adhesion was determined after 2 h. Colors correspond to the different EpaA subtypes as shown in Fig. 3A. (B) Pie charts show glycan types of the CFG array for which binding signals exceed either 20% or 50% of the signal of the best binder. For each glycan type (color as above), the core structures are shown below in CFG standard notation. Nonassigned glycans are grouped as “other sugars” (yellow).