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. 2012 Sep 14;109(42):E2856–E2864. doi: 10.1073/pnas.1208509109

Fig. 3.

Fig. 3.

3HP inhibits the growth of E. coli K12 on M9/glucose. (A) Growth of the ΔpdxB strain with 10 μM pyridoxine (●), 0.5 mM 3HP (red △), or 1.0 mM 3HP (○). (B) Growth of WT E. coli with no supplement (●) or with 0.2 mM 3HP (yellow □), 0.5 mM 3HP (red △), or 1.0 mM 3HP (○). (C) Growth of replicate cultures of WT E. coli K12 on M9/glucose. 3HP (final concentration of 1 mM) was added to one culture (○) at the time indicated by the first arrow. After 90 min (second arrow), supplements were added to give the following final concentrations: 1 mM Ser and 40 μM Ile (yellow ○), 1 mM Ser (□), or 40 μM Ile (blue □). (D) Growth of WT E. coli K12 on M9/glucose with no supplement (●); with 1.0 mM 3HP (○); or with 1.0 mM 3HP, 1 mM Ser, and 40 μM Ile (yellow ○). A = OD600.