Fig. 2.

Single-channel currents of KvLm recorded in DIBs. (A) Single-channel currents of KvLm FL₄ and PM₄ homotetramers produced by IVTT expression or (B) by E. coli expression, after reconstitution in DIBs. Single-channel currents were recorded at V = +150 mV and V = -150 mV. c and o denote closed and open. (C) Single-channel all-points current histograms of FL₄ (Left) and PM₄ (Right) expressed either by IVTT (red) or in E. coli (black). Currents were recorded at V = +150 mV. γ of FL₄ expressed by IVTT = 73 ± 7 pS, and that of PM₄ = 113 ± 7 pS. γ of FL₄ expressed in E.coli = 66 ± 7 pS, and that of PM₄ = 105 ± 10 pS.