Fig. 4.
Permeation and gating properties of KvLm tetramers. (A) I–V curves of KvLm homotetramers and heterotetramers. FL4 did not open at negative potentials. (B) Open occupancy (Po) curves for homotetramers and heterotetramers. The smooth curves represent the best fit of the Boltzmann function to the data. (C) Increase in apparent gating charge with the number of voltage sensors in a tetramer. (D) Po (red squares) and median burst duration (black squares) as functions of the number of voltage sensors in the assembly. V = +150 mV. Error bars represent SEM; the number of experiments for each tetrameric assembly was ≥4.