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. 2012 Oct 1;109(42):16841–16846. doi: 10.1073/pnas.1215406109

Fig. 1.

Fig. 1.

WNK3 binds to mRNA processing factors. (A) List of mRNA processing factors identified as WNK3-binding partners from yeast two-hybrid screens. (B) FLAG-tagged Fox-1 (F:Fox-1) was expressed in HEK293 cells with or without Myc-tagged proteins as indicated. The protein complex was coimmunoprecipitated with anti-Myc antibody and analyzed by immunoblotting. (C) Myc-tagged proteins were expressed in HEK293 cells and anti-Myc antibody was used for coimmunoprecipitation to detect bound endogenous Fox-2. In B and C, the arrow marks the position of F:Fox-1 or endogenous Fox-2, respectively. In both, the asterisk represents the position of IgG. (D) Transfection and coimmunoprecipitation were as described in B and proteins were immunoblotted. (E and F) Proteins were expressed in HEK293 cells as indicated and then immunoprecipitated with M2 (anti-FLAG antibody) resin followed by immunoblotting. Alpha-tubulin was used as a loading marker. (G) HEK293 cells were lysed and immunoprecipitated with anti-WNK3 antibody. Associated proteins were detected by immunoblotting with the indicated antibodies.