Fig. 2.

Mapping the interacting regions on WNK3 and Fox-1. (A) Schematic representation of protein domains in human WNK3 (Left). Shaded regions indicate protein domains including Kinase, AI (auto inhibitory), and CC (coiled-coil) domains. Outside these regions, WNKs are poorly conserved. Numbers are the amino acid residues of full-length protein or the boundaries of small fragments linked to GST. GST pull-down assays with bacterially purified F:Fox-1 are presented (Right). (B) Schematic view of mouse Fox-1. RRM = RNA recognition motif. Fragments were generated for WNK3 interaction assays and binding results are summarized on the right. (C) Coimmunoprecipitation of Myc-WNK3 (1474–1743) with FLAG-tagged Fox-1 fragments from HEK293 cells.