Fig. 1.

P7C3A20 and P7C3 block motor neuron cell death in the spinal cord when administered at the time of disease onset to G93A-SOD1 mutant mice. Treatment of G93A-SOD1 mutant mice with 20 mg⋅kg⁻¹⋅d⁻¹ of P7C3A20, P7C3, or Dimebon, or the appropriate vehicle, was initiated on day 80. Five mice from each group were killed on days 90, 100, 110, and 120. The number of spinal cord motor neurons per cubic millimeter of lumbar spinal cord was determined through immunohistochemical staining for ChAT and quantification with National Institutes of Health Image J software. All images were analyzed blind to treatment group. Spinal cord motor neurons in G93A-SOD1 mutant mice died over time as expected. Spinal cord motor neuron cell death was blocked by administration of P7C3A20. P7C3 was intermediately protective, whereas Dimebon had no neuroprotective efficacy. (Upper) Representative staining of ChAT of one ventral horn from each of the five mice in each treatment group at 110 d is shown above the graph.