Fig. 1.

Testing isogenic HSV and PRV XFP-NLS viruses. Three isogenic viruses for either HSV-1 strain 17 (A–D) or PRV (E–H) were tested for replication and anterograde directed spread. (A) Vero cells or (E) PK15 cells were infected with individual HSV or PRV strains at a MOI of 10 and harvested at 0, 6, 12, and 24 h postinfection. Viral titers were determined by limiting dilution on their respective cell lines. (B and F) Representative images taken with a 20× magnification objective of a MOI 100 infection with a mixture of the three fluorophore expressing strains used to calculate the maximum average genome expression value (Table 1). (C, D, G, and H) Individual viruses and a mixture of all three viruses were used to infect the neuronal cell bodies. (C and G) Viral titers from neuronal cell bodies or (D and H) isolated axon extensions in compartmentalized neuronal culture harvested 48 and 24 h postinfection, respectively.