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. 2012 Sep 17;109(40):16101–16106. doi: 10.1073/pnas.1211023109

Fig. 2.

Fig. 2.

In vitro cytotoxicity assays. (A) Anti–Her2-IgG(HC-A121X)-nAF (EC50 0.37 ± 0.38 nM) is more cytotoxic than the unconjugated auristatin-linker (EC50 1.5 ± 1.7 nM) and almost 100-times more cytotoxic than Fab(LC-K169X)-nAF (EC50 21.3 ± 13.7 nM) on MDA-MB-435/Her2+ cells. (B) IgG(HC-A121X)-nAF has little effect on MDA-MB-435/Her2− cells but the Fab-nAF is cytotoxic at >40 nM. The EC50 of the auristatin-linker alone on MDA-MB-435/Her− cells is 0.92 ± 2.4 nM. (C) The cytotoxicity of nAF conjugated to sites LC-S202X (EC50 2.1 ± 2.8 nM) and HC-A121X (EC50 1.8 ± 0.33 nM) on the Fab were slightly greater than the cytotoxicity of Fab(LC-K169X)-nAF (EC50 8.3 ± 3.4 nM) on SK-BR-3 cells. Percent viability is normalized between no compound and 5 μM taxol controls. Error bars represent SD of three replicates.