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. 2012 Sep 17;109(40):16143–16148. doi: 10.1073/pnas.1208253109

Fig. 4.

Fig. 4.

Altered expression and function of integrin αIIb and other platelet glycoproteins in Cosmc-deficient platelets. (A) Fluorescent microscopy: EHC Cosmc+/y and EHC Cosmc−/y platelet binding to fibrinogen detected by fluorescein-labeled phalloidin; EHC Cosmc+/y platelets without (A-1) or with (A-3) thrombin stimulation. EHC Cosmc−/y platelets without (A-2) or with (A-4) thrombin stimulation. SEM: thrombin-stimulated EHC Cosmc+/y and EHC Cosmc−/y platelets (A-5 and A-6). (B and C) In the absence of Cosmc, thrombin fails to stimulate activated αIIbβ3 measured by JON/A antibody (B) and P-selectin expression on the surface of platelets (C). Comparing resting to thrombin-stimulated platelets, *P = 0.0388, **P = 0.0219 (B), *P = 0.0102, **P = 0.00037 (C), NS, not significant as noted in graph (*, unequal variance; NS and **, equal variance). (D) Western blots of EHC Cosmc+/y and EHC Cosmc−/y platelets with anti-GPIIb and anti-GPIIIa antibodies. (E) Western blot of GPVI in platelet lysates in reducing conditions.