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. 2012 Aug 20;109(40):16113–16118. doi: 10.1073/pnas.1209615109

Fig. 2.

Fig. 2.

The Ndc80 complex slows microtubule disassembly and stabilizes protofilament extensions. Kymographs of disassembling microtubules (red) in the presence of (A) 100 pM or (B) 500 pM GFP-tagged Ndc80 complex (green). Brightness and contrast were adjusted equally in A and B. (C) Mean disassembly speeds ± SEM for microtubules in the presence of increasing concentrations of Ndc80 complex (without Ndc80 complex, n = 80; 100 pM Ndc80 complex, n = 31; 250 pM, n = 29; 500 pM, n = 34). (D) Time-lapse images of a disassembling microtubule (red) in the presence of 500 pM GFP-tagged Ndc80 complex (green) as a curled extension formed at the tip. Inset numbers show elapsed time, in seconds. See Fig. S3B for a gallery of images showing curled extensions. (E) Negative-stain electron micrograph of a disassembling microtubule tip (see SI Materials and Methods) stabilized by the Ndc80 complex. An arrow marks the transition from a closed microtubule to an open sheet. The figure was constructed from three images, the boundaries of which are depicted by dotted white lines.