Fig. 5.

Identification of Topo VI binding sites in vivo. ChIP assays were carried out using anti-GFP and anti-HA antibodies with wild-type (Col), rhl1-2 RHL1-CFP, and rhl2-1 HA-RHL2 plants that were grown for 6 d in low light (LL; 12 μmol⋅m⁻²⋅s⁻¹) and then transferred to moderate high light (HL; 1,050 μmol⋅m⁻²⋅s⁻¹) for 30 min. PCR analysis was carried out to probe RHL1 and RHL2 binding to the promoter region of the ¹O₂-responsive AAA-ATPase, At1g24145, and At1g24147 genes and the control locus At4g07360. Filled boxes indicate position and size of PCR-amplified fragments. The experiment was repeated three times with comparable results.