Fig. 1.

Ppi interference with phage 80α. (A) Approximately 10⁸ bacteria were infected with phage 80α, plated on phage bottom agar, and incubated 36–48 h at 32 °C. Plates were stained with 0.1% TTC in TSB and photographed. Genes cloned into pCN51 were induced with 1.0 μM CdCl₂. (B and C) Electron microscopic analysis of sedimented particles from (B) phage 80α lysate of RN4220 + pCN51 and from a (C) 80α lysate of RN4220 + pCN51 – ppi_spb2. CP, complete phage 80α particles; FT, free phage tails; PH, phage proheads.