Table 1.

Kinetic parameters of recombinant PhCHD

Substrate	Km, μM	Vmax, μmol·min−1·mg−1	kcat, s−1	kcat/Km, mM−1·s−1
Cinnamoyl-CoA*	89.8 ± 1.8	3.25 ± 0.01	4.35 ± 0.01	48.5 ± 0.9
p-coumaroyl-CoA*	73.7 ± 3.3	0.30 ± 0.01	0.40 ± 0.01	5.5 ± 0.2
NAD+†	13.8 ± 0.7	5.19 ± 0.06	6.94 ± 0.08	506.2 ± 20.6
NAD+‡	6.1 ± 0.2	0.18 ± 0.00	0.24 ± 0.00	39.5 ± 1.4

Values are means ± SEM (n = 3).

*[NAD⁺] 1mM.

^†[Cinnamoyl-CoA] 0.4 mM.

^‡[p-coumaroyl-CoA] 0.4 mM.