Table 1.
Substrate | Km, μM | Vmax, μmol·min−1·mg−1 | kcat, s−1 | kcat/Km, mM−1·s−1 |
Cinnamoyl-CoA* | 89.8 ± 1.8 | 3.25 ± 0.01 | 4.35 ± 0.01 | 48.5 ± 0.9 |
p-coumaroyl-CoA* | 73.7 ± 3.3 | 0.30 ± 0.01 | 0.40 ± 0.01 | 5.5 ± 0.2 |
NAD+† | 13.8 ± 0.7 | 5.19 ± 0.06 | 6.94 ± 0.08 | 506.2 ± 20.6 |
NAD+‡ | 6.1 ± 0.2 | 0.18 ± 0.00 | 0.24 ± 0.00 | 39.5 ± 1.4 |
Values are means ± SEM (n = 3).
*[NAD+] 1mM.
†[Cinnamoyl-CoA] 0.4 mM.
‡[p-coumaroyl-CoA] 0.4 mM.