Table 2.

Enrichement of PhCHD activity in peroxisomes

		Marker assay
Sample	PhCHD*	Catalase†	ADH*	Fumarase†	Chlorophyll‡
Crude extract	131 ± 11	58 ± 2	125 ± 13	342 ± 34	0.16 ± 0.01
Peroxisomes	1472 ± 111	458 ± 54	<1	262 ± 53	<0.01

PhCHD and marker enzyme activities were assayed in crude extracts and percoll-purified peroxisomes from petunia corollas sampled at 8:00 PM on day 2 postanthesis. Catalase, alcohol dehydrogenase (ADH), and fumarase were used as marker enzymes for peroxisomes, cytosol, and mitochondria, respectively, whereas chlorophyll content was used as the plastidic marker. Data are means ± SEM (n = 3).

*pkat·mg⁻¹.

^†nkat·mg.

^‡ng·g FW⁻¹.