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. 2012 Sep 17;109(40):16383–16388. doi: 10.1073/pnas.1211001109

Table 2.

Enrichement of PhCHD activity in peroxisomes

Marker assay
Sample PhCHD* Catalase ADH* Fumarase Chlorophyll
Crude extract 131 ± 11 58 ± 2 125 ± 13 342 ± 34 0.16 ± 0.01
Peroxisomes 1472 ± 111 458 ± 54 <1 262 ± 53 <0.01

PhCHD and marker enzyme activities were assayed in crude extracts and percoll-purified peroxisomes from petunia corollas sampled at 8:00 PM on day 2 postanthesis. Catalase, alcohol dehydrogenase (ADH), and fumarase were used as marker enzymes for peroxisomes, cytosol, and mitochondria, respectively, whereas chlorophyll content was used as the plastidic marker. Data are means ± SEM (n = 3).

*pkat·mg−1.

nkat·mg.

ng·g FW−1.