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The network of the three-pronged IP–MS analysis. (A) The PPI network integrating data from three separate IP-LC-MS/MS experiments using endogenous antibodies to p85 (protein capture) and GRB2 (protein capture) as bait proteins and pTyr (peptide capture) to identify the active tyrosine kinase signaling pathways in H929 MM plasma cells. (B) Venn diagram showing the overlap of proteins/peptides from each IP–MS experiment.
