Skip to main content
. 2012 Sep 17;109(40):16190–16195. doi: 10.1073/pnas.1212759109

Fig. 2.

Fig. 2.

Evidence for BCR–ABL signaling in H929 MM cells. (A) PathScan RTK signaling antibody array kit tests the phosphorylated signal level of kinases and key signaling nodes. After serum starvation, H929 MM cells were stimulated for 90 min with insulin, insulin growth factor (IGF), epidermal growth factor (EGF), or 10% FCS or were serum starved overnight. (B) Immunoblots of the MM cell lines OCI-MYC, KMS 12BM, IIN3, XG7, U266, RPMI-8226, MOLP8, H929, and K562 CML to test for the BCR–ABL fusion protein from whole cell lysates (WCL). (C) ABL IP is immunoblotted for BCR to check for the presence of the BCR–ABL fusion in H929 cells.