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. 2012 Sep 17;109(40):16342–16347. doi: 10.1073/pnas.1205838109

Fig. 3.

Fig. 3.

Gi coupling properties of ∆2∆ reconstituted into nanodiscs. (A) Kinetics of GTPγS binding to Gi in Δ2Δ nanodiscs treated with 10 μM LY487379 (PAM) or 10 μM MNI-136 (NAM). Data were analyzed as described in SI Materials and Methods. Data points represent the mean ± SEM of triplicate determinations from a representative experiment out of three. (B) Dose–response of LY487379 and MNI-136 on Δ2Δ nanodiscs. Values correspond to the fractional GTPγS binding given by (Δ − Δref)/(Δmax − Δref), where Δref is the It40 − It0 value in the absence of ligand for the PAM curve or in the presence of 10 μM PAM for the NAM curve. (C) GTPγS binding (It40 − It0 value) measured with empty nanodiscs, nanodiscs ∆2∆ WT, or ∆2∆ F756P in the presence of 10 μM PAM. Data points in B and C represent the mean ± SEM of three independent experiments.