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. 2012 Sep 17;109(40):16202–16207. doi: 10.1073/pnas.1208533109

Fig. 4.

Fig. 4.

Loss of Zfp281 facilitates somatic cell reprogramming. (A) Summary of the procedure for iPSC generation. (B) RT-qPCR analyses of Zfp281 and Nanog expression during iPSC generation. Expression levels were normalized to those in wild-type ESCs. (C) RT-qPCR analyses of relative Nanog expression during iPSC generation upon knockdown with control scramble (SCR) or Zfp281 shRNA. Expression levels were normalized to those in wild-type ESCs. (D) Minimal reduction of total AP (+) colony numbers upon Zfp281 knockdown during reprogramming. (E) Zfp281 knockdown promotes iPSC generation. Oct4-GFP MEFs were infected with viruses expressing the four reprogramming factors (4F), alone (–) or together with three independent shRNAs against Zfp281 (1–3) and control scramble shRNA (shSCR). The same reprogramming assays were repeated independently three times (A, B, and C) with duplicates each time (1, 2). The average percentages of GFP (+) and GFP (−) colonies from three independent experiments are shown in the pie chart (Upper).