Figure 3.

Critical residues in the PC5A processing of NCAD. (A) HeLa cells were co-transfected with cDNAs coding for PC5A and either WT NCAD or its P1, P4, and P6 mutants. A control with no PC5A expression was included. Twenty-four hours after transfection, the media were analyzed by Western blot using the proN Ab. The migration positions of the NCAD prodomain with apparent molecular masses of ∼17 and 20 kDa are emphasized. (B) In a similar fashion, the PC5A processing of NCAD mutants at specific Asn or Ser residues is shown.