Figure 6.

Effects of STAT5b blockade in an orthotopic tumor model. (A) Orthotopic implantation of HPAF-II pancreatic cancer cells into the pancreatic tail of nude mice results in inhibition of tumor growth after 31 days, as determined by final tumor weight (*P < .05). (B) STAT5b knockdown was persistent after 31 days (Western blot analysis). (C) Blockade of transcription factor STAT5b leads to a significant reduction in tumor cell proliferation (BrdU incorporation; *P < .05). (D) No effect was observed on tumor cell apoptosis (TUNEL staining). (E) However, tumor vascularization, measured by CD31 vessel area, was reduced on STAT5b inhibition (*P < .05). Bars, SEM.