Selection of a cell line that represents B-TNBC. MCF-7, MDA-MB-231, and HCC1806 were screened by endpoint RT-PCR for expression of (A) hormone receptors and HER2, (B) luminal cytokeratins, (C) basal cytokeratins, and (D) vimentin, a mesenchymal marker. (E) Basal markers and vimentin were confirmed by RT-qPCR and normalized to β-actin presented as dCt ± SE. (F) Protein expression of basal cytokeratins 5, 6, and 14 was also confirmed by Western blot analysis in which β-actin demonstrated equal protein loading.