Figure 6.

XPO1-dependent ARID1A nuclear export. (A) ES2 cell lysate was immunoprecipitated using a rabbit anti-XPO1 antibody or normal rabbit IgG. Total cell extracts (input) and immunoprecipitates were then probed using antibodies against XPO1 or ARID1A followed by a mouse anti-rabbit conformation-specific antibody. (B) Nuclear and cytoplasmic levels of ARID1A were determined in OSE4 cells transfected with wild-type ARID1A. Sixteen hours after transfection, cells were treated with leptomycin B (LMB) for an additional 24 hours; during the last 6 hours of incubation, MG132 was added to some of the cells as indicated. Protein levels of ARID1A-V5 were detected by Western blot analysis with an anti-V5 antibody. GAPDH and p53 served as loading controls for cytoplasmic and nuclear protein fractions, respectively.