| Master Mix | Final Conc | Volume (μL) |
| H20 PCR | 2.875 | |
| Quantitect RT-PCR Master Mix | 1x | 6.250 |
| RT Mix | 1x | 0.125 |
| 50 μM Cog R primer | 1 μM | 0.250 |
| 50 μM Cog F primer | 1 μM | 0.250 |
| 10 μM Ring probe | 1 μM | 0.125/0.250* |
| 10.00 |
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