Master Mix | Final Conc | Volume (μL) |
H20 PCR | 29.50 | |
5X Qiagen RT-PCR Buffer | 1x | 10.00 |
10 mM dNTP Mix | 0.4 mM | 2.00 |
Enzyme Mix | 2.00 | |
40 U/μL RNAsin | 20 U/μL | 0.50 |
10 μM SKF | 0.1 μM | 0.50 |
10 μM SKR | 0.1 μM | 0.50 |
45.00 |
Master Mix | Final Conc | Volume (μL) |
H20 PCR | 29.50 | |
5X Qiagen RT-PCR Buffer | 1x | 10.00 |
10 mM dNTP Mix | 0.4 mM | 2.00 |
Enzyme Mix | 2.00 | |
40 U/μL RNAsin | 20 U/μL | 0.50 |
10 μM SKF | 0.1 μM | 0.50 |
10 μM SKR | 0.1 μM | 0.50 |
45.00 |