| Step | Temp (°C) | Time (min) | |
| 1 | 60 | 30:00 | cDNA synthesis |
| 2 | 96 | 15:00 | HotStart Taq polymerase activation |
| 3 | 94 | 00:30 | |
| 52 | 01:00 | 40X cycles | |
| 72 | 00:30 | ||
| 4 | 72 | 10:00 | Annealing |
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