Figure 1.

Histology of liver slices in culture. Histology of transversal (a, b) and longitudinal sections (c–f) of rat liver slices immediately after preparation (a) and after 24 (b, c), 48 (d), 72 (e), and 96 h (f) of culture. Hematoxylin and eosin staining illustrates the integrity of liver architecture after slicing (a) and the appearance of a thin necrotic area at the center of the slice ((b), dark arrow). (c–f), semithin sections stained with azur blue evidenced the apoptotic bodies at 48 h ((d), white arrow), the microvacuoles in hepatocytes at 72 h ((e), white arrow) and disorganized periportal zone at 96 h ((f), dark arrow); bar 100 μm.