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. 2012 Oct 16;2012:785786. doi: 10.1155/2012/785786

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Copyright © 2012 Francis Finot et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course of caspase expression and activation. (a), Western blot analysis of caspase 8, 9 and 3 in normal liver (NL), core (C), after slicing (Sl), after pre-incubation (T0) and at the indicated times of culture. IETD- (b), LEHD- (c) and DEVD-AMC (d) caspase activities were measured in cell lysates from slices (open circles) and isolated hepatocytes (dark triangle) at different times of culture. Activities were expressed in arbitrary units (A.U.) of fluorescence. Caspase activities in isolated hepatocytes were measured in 6 independent experiments while activities in liver slices are the results of 3 independent experiments with 2 or 3 slices in each experiment. *P < 0.001 treatment versus control.