Figure 2.

To measure proliferation, the MTS assay was performed at selected times on cultures of astrocytes from retina and optic nerve of wild type and Nuc1 rats. The data represent the mean value of the absorbance at λ = 490 nm, which is proportional to the number of viable cells. Experiments were done in triplicate. P-values were calculated between Nuc1 mutant and wild type cells using Student t-test (* P = 0.03, *** P <0.0007).