Fig. 4.

Akt is activated during proestrus and inactivated during estrus. (A) Images of peroxidase labeling of phosphorylated Akt in the dorsal hippocampal formation of representative sections from one proestrus and one estrus mouse. pAkt-ir is darker throughout the hippocampus inproestrus than in estrus. (B) Quantification of pAkt-ir in four hippocampal subregions across the estrus cycle. pAkt-ir was significantly higher in proestrus compared with estrus (P<0.0001), and in diestrus compared with estrus (P<0.0001). From (Spencer et al 2008) by permission.