Table 2.
Crystallographic data statistics from the analysis of lysozyme crystals.
| Parameter | On-Chipa | Traditional (Cryogenic)b |
|---|---|---|
| Unit Cell Dimensions | a = b = 79.693Å c = 37.781Å |
a = b = 78.817Å c = 37.025Å |
| Space Group | P43212 | P43212 |
| Observations (Unique) | 783,994 (18,352) | 223,433 (17,510) |
| Resolution | 50 – 1.55Å | 50 – 1.55Å |
| Rsym | 0.064 (0.362) | 0.052 (0.102) |
| Mosaicity | 0.03° – 0.08° | 0.21° – 0.34° |
| Redundancy | 22.9 (5.7) | 7.7 (7.7) |
| Completeness | 98.1% (83.4%) | 99.7% (100%) |
| I/σ | 51.4 (3.9) | 42.3 (19.4) |
| # of Frames | 363 | 100 |
|
Refinement | ||
| R (Rfree) | 0.164 (0.227) | 0.173 (0.276) |
|
Ramachandran Statistics | ||
| Most Favored | 96.1% (122) | 96.1% (122) |
| Allowed | 3.9% (5) | 3.9% (5) |
| Disallowed | 0.0% (0) | 0.0% (0) |
Merging of small datasets from "multiple crystals" analyzed on-chip within a 24-well device at room temperature.
The "traditional" sample was grown using microbatch techniques and mounted using a standard crystal mount for cryogenic data collection. Reported values are for all hkls. Values in parenthesis represent the value for the highest resolution shell except where indicated for the number of observations as compared to unique reflections and R (Rfree) and for the Ramachandran statistics here the number in parenthesis indicates the number of residues in a given region. Data was analyzed over the range of 50 – 1.55Å to enable a direct comparison with the data collected from merging the diffraction data taken from multiple crystals at RT.