Fig. 5.

Preferential binding of WbHMGB1 protein to DNA and super-coiled DNA. a Gel retardation experiments were carried out with mixture of supercoiled DNA and closed-circular DNA. Increasing amount of His-tagged WbHMGB1 (0.5–5 μM, left to right) was added to determine the binding. b An equimolar mixture of supercoiled and linearized plasmid was pre-incubated with increasing amounts of WbHMGB1 (0.1–2 μM, from left to right). DNA–protein complexes were resolved on 1 % agarose gel, followed by staining the gel with ethidium bromide. S supercoiled DNA, L linear DNA, C relaxed open circular DNA