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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Microbiol. 2012 Jan 9;83(3):612–622. doi: 10.1111/j.1365-2958.2011.07956.x

Fig. 1.

Fig. 1

Identification of LEE1 P1 transcription initiation site. (A) Map of plasmid carrying LLE1 P1 promoter DNA from −200 to +80 in pSA508(Choy & Adhya, 1993), designated pHJ12. ter is a factor-independent transcription terminator. (B) Lanes 1 to 4 show in vitro transcription reaction with supercoiled plasmid pHJ101 (SC) and with the plasmid linearized with EcoR1, BamH1, and Pst1, respectively. The reaction products were analyzed by 8 M urea/6% polyacrylamide gel electrophoresis. The major transcripts from LEE1 P1 and rna1 are indicated.