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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Microbiol. 2012 Jan 9;83(3):612–622. doi: 10.1111/j.1365-2958.2011.07956.x

Fig. 2.

Fig. 2

Analysis of the multiple transcripts from the LEE1 P1 promoter. (A) RNA generated from in vitro transcription reaction using pHJ12 as template (in vitro) and from E. coli (MG1655) carrying pHJ12 (in vivo) were determined by primer extension analysis using a primer annealing to +54~+74. (B) In vitro transcription reaction carried out with α32P UTP or γ32P ATP as indicated. (C) Transcription initiation points, shown as underlined As, near the previously assigned +1G (shown in bold).