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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Microbiol. 2012 Jan 9;83(3):612–622. doi: 10.1111/j.1365-2958.2011.07956.x

Fig. 3.

Fig. 3

(A) In vitro transcription with wild-type LEE1 P1 (WT) and mutants carrying A to G substitutions close to the previously assigned +1G, as describe in the text. The assay was carried out with variant pSA508::LEE1 P1 (−200 to +20), and the reaction products were analyzed by 8 M urea/10% polyacrylamide gel electrophoresis. (B) tsp for P1A and P1B are indicated. The newly assigned +1A is underlined, and the putative −10 hexamers for P1A and P1B are shown in bold. −35 hexamer for P1A predicted by sequence walker (Supporting Information) is indicated.