Fig. 1.

Osteopontin induces the migration activity of glioma cells. By using a cell culture insert system, in vitro migration activities were examined. (A) After incubating cells with various concentrations of osteopontin for 24 h, we found that osteopontin induced migration activity in U251 and C6 cells. Results are expressed as means ± SEM of 3 independent experiments. (B) Cells were treated with various concentrations of osteopontin for 24 h. The migrated cells were visualized by phase-contrast imaging. (C) Cells were seeded on the migration insert for 24 h and treated with or without osteopontin (10 ng/mL) for another 24 h. The migrated cells were determined by wound-healing assay and visualized by phase-contrast imaging. (D) Treatment with osteopontin (10 ng/mL) enhanced invasion of U251 cells. Results are expressed as means ± SEM of 3 independent experiments. *P < .05 compared with control group.