Fig. 2.

Osteopontin increases HO-1 upregulation in U251 glioma cells. Cells were stimulated with osteopontin with various concentrations (1, 3, or 10 ng/mL for 24 h; A) or with a concentration of 10 ng/mL for indicated time periods (4, 8, and 24 h; B). After cell lysate extracts were collected, HO-1 protein levels were determined by using Western blot analysis. (C) Cells were treated with osteopontin (10 ng/mL), and HO-1 mRNA expression was analyzed by RT-PCR. (D) mRNA expression was quantitated by real time–PCR. Results are expressed as means ± SEM of 3 independent experiments. *P < .05 compared with control group.