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. 2012 Nov;14(11):1367–1378. doi: 10.1093/neuonc/nos262

Fig. 3.

Fig. 3.

Osteopontin-directed glioma cell migration involves HO-1 expression. (A and B) Cells were treated with ZnppIX (0.1 or 0.3 μM) for 30 min, followed by stimulation with osteopontin (10 ng/mL). (C) Cells were stimulated with CoPPIX for 24 h. (D, lower panel) Cells were pretransfected with control or HO-1 siRNA for 24 h, followed by stimulation with osteopontin (10 ng/mL) for another 24 h. In vitro migration activities were examined after osteopontin treatment for 24 h. Results are expressed as means ± SEM of 3 independent experiments. *P < .05 compared with the control group; #P < .05 compared with the osteopontin treatment group. Cells were pretransfected with control or HO-1 siRNA for 24 h, and protein expression was determined by Western blot (D, upper panel).