Fig. 7.

High migration activity of human glioma cells correlated with cell invasiveness in vivo. After 10 rounds of selection of U251 cells by a cell culture insert system, the migration-prone subline (P10; B) and the original group (P0; A) were implanted into nude mice intracranially at the site indicated as a; mice were killed 28 days after implantation, and representative sections were stained with hematoxylin. Scale bar = 200 μm in A, B. Higher magnification of the migration-prone subline P0 are shown in b. Higher magnification of the migration-prone subline P10 is shown in c, d, e, and f (n = 3 mice in each group). Scale bar = 100 μm in b–f.