Figure 2.

wiry Mutants Are Impaired in Genes of the ta-siRNA Biogenesis Pathway.

(A) to (D) A scheme of the tomato orthologs of RDR6 (A), AGO7 (B), SGS3 (C), and DCL4 (D) and the lesions identified in the different alleles. aa, amino acids; dsRBM, double-stranded RNA binding motif.

(E) and (F) Different miRNAs, such as miR166, miR164, and miR390, are present in both wild-type (WT) and wiry shoots (E), but the ta-siARF derived from TAS3 could not be detected in any of the four wiry mutants (F).

[See online article for color version of this figure.]