Figure 4.

Small RNAs Derived from the ARF4 and ARF3 Transcripts.

(A) In the wild type (WT), ARF4-derived siRNAs (i) originate mainly from the sequence flanked by the two ta-siARF recognition sites (red arrows), and ARF3-derived siRNAs (ii) are present in low numbers and originate from all parts of the mRNA.

(B) More ARF4-derived siRNAs were found in dcl4 apices (i) as well as siRNAs from the gene’s 3′ end but not from ARF3 (ii).

(C) The ARF4-derived siRNAs (i) are 20 to 22 nucleotides in the wild type and primarily 22 to 24 nucleotides in dcl4 but the ARF3-derived siRNAs (ii) are 20 to 21 nucleotides in the wild type and primarily 22 nucleotides in dcl4.

In (A) and (B), the x axis marks the position along the gene, but in (C), it marks the small RNA size. The y axis marks the numbers of reads.