Figure 5.

Normal and DCL4-Independent Production of TAS3 siRNAs.

(A) Wild-type (WT) small RNAs derived from the TAS3-1 (i), TAS3-7 (ii), and TAS3-12 (iii) genes. Most small RNAs are derived from the fragment flanked by the miR390 recognition sites (red arrows).

(B) In the dcl4 library, small RNAs from TAS3-1 (i) and TAS3-7 (ii) are present, but small RNAs from TAS3-12 are not present (iii).

(C) Size distribution of small RNAs derived from the TAS3 genes. In the wild type, most are 21 nucleotides, but in the dcl4, they are shifted to 22 nucleotides.

In (A) and (B), the x axis marks the position along the gene, but in (C), it marks the small RNA size. The y axis marks the numbers of reads.