Figure 1.

Arabidopsis PDGLP1 and PDGLP2 Are Plasmodesmal Targeted Proteins.

(A) Phylogenetic analysis of the relationship between the Arabidopsis GLP family and the tobacco PDGLP1 identified in our tobacco PECP co-IP studies using the pumpkin phloem Cm-PP16 as bait. The phylogenetic tree was constructed in MEGA 3.1; numbers at each branch point represent the bootstrap values for percentage of 1000 replicate trees.

(B) PDGLP1 (At1G09560) and PDGLP2 (At1G02335) are colocalized with the PD marker CMV MP-GFP. The C-terminal mCherry (RFP) tagged At1G09560, At1G02335, and At1G18970 (GLP4) were agroinfiltrated into leaves of CMV MP-GFP transgenic N. benthamiana plants. Yellow signal in merged images represents colocalization of At1G09560 and At1G02335 with CMV MP-GFP. Images were taken by CLSM. Bars = 10 µm.

(C) PDGLP1-GFP is located within PD. Leaf and root tissues of PDGLP1-GFP transgenic plants were processed for transmission electron microscopy–based double immunogold labeling with anticallose Ab (15-nm gold particles) and anti-GFP Ab (10-nm gold particles). Red arrowheads indicate detection of PDGLP1-GFP within the PD. Bar = 200 nm.

[See online article for color version of this figure.]