Figure 2.

DYW1 Is Localized in Chloroplasts of Arabidopsis Cells.

(A) Fluorescence images of Arabidopsis plantlets transiently expressing DYW1-RFP fusion proteins. Either the first 100–amino acid polypeptide or the full-length DYW1 protein, starting both at the M₂ Met encoded by the ATG₂ codon, were expressed as a fusion with the RFP at the C terminus. Plantlets were observed by confocal microscopy 4 d after transformation. Bars = 10 μm.

(B) Immunoblot analysis of total extract (T), mitochondrial (M), and chloroplast (CP) protein fractions with antibodies directed against DYW1. Protein fractions from Arabidopsis were analyzed by immunoblots. Loading control (Coomassie blue staining; left panel) shows that equal amounts of total protein were loaded. Black bars represent molecular mass marker positions. The purity of the two fractions was tested with antibodies directed against the mitochondrial formate dehydrogenase (FDH) and the chloroplast large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo).