Figure 5.

In Planta Protein Interaction of DYW1 and CRR4 as Shown by BiFC.

BiFC of YFP in transiently transformed Arabidopsis plantlets. Left column, YFP fluorescent signal detection by confocal microscopy; middle column, chlorophyll autofluorescence; right column, merge of fluorescent signal and autofluorescence. Cotransformation of Arabidopsis plantlets with DYW1-nYFP and CRR4-cYFP (top lines) generates yellow fluorescence that colocalizes with autofluorescence of chlorophylls in plastids. Similar signals are observed when using the homodimerization of MinD1 (third panel) as a positive control (Maple et al., 2007). No signal was observed when plantlets were cotransformed with either DYW1-nYFP and MinD1-cYFP or DYW1-nYFP and CLB19-cYFP (bottom panels). Bars = 6 μm.